Thermo Q Exactive ™ Combination quadrupole Orbitrap ™ mass spectrometer

Using Thermo Scientific ™ Q Exactive ™ The combination quadrupole Orbitrap mass spectrometer can quickly identify, quantify, and confirm more compounds. This desktop LC-MS/MS system combines quadrupole mother ion selection with high-resolution accurate mass number (HRAM) Orbitrap detection, providing stable performance and versatility. The Q Exactive mass spectrometer is suitable for screening non target or target compounds, and can also achieve qualitative and quantitative applications. It can be used for drug invention, proteomics, environment and food, clinical research, and forensic toxicology.

Analysis of genotoxic impurities in drugs

The US Food and Drug Administration (FDA) has released a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method for quantitative determination of N-nitrosodimethylamine (NDMA) in ranitidine raw materials and formulations using a Q Exactive combination quadrupole Orbitrap mass spectrometer.

HRAM and full scan capability can capture all sample data from start to finish, enabling retrospective data analysis without the need for rescanning the samples

The resolution reaches 140000 FWHM, which can eliminate the interference of compounds with the same amount but different sequences and isomers, and improve the stability of results when analyzing samples in complex matrices

The quality accuracy of both full scan and full ion lysis (AIF) modes is better than 1 ppm, ensuring reliable compound identification. The rapid scan and spectral multi-channel technology at 12 Hz are particularly suitable for UHPLC applications

Quick polarity switching can obtain as much information as possible

AIF and various dissociation techniques - in source CID and HCD - can assist in compound identification

The extended mass range reaches 6000 m/z, which can improve the detection ability of single charged small molecules and biomolecules. With a scanning dynamic range of over four orders of magnitude and Flyke level sensitivity, it can simultaneously detect trace level and high abundance compounds in one scan, making it more suitable for drug invention and metabolomics research

The quantitative capability equivalent to triple quadrupole mass spectrometry can not only be used for quantitative analysis of parent compounds and metabolites, but also provide higher throughput for identifying and determining compounds. The fast HRAM Orbitrap technology can eliminate interference, achieve metabolite structure identification, and perform large-scale spectral analysis of metabolomics and lipidomics samples. Both MS and MS/MS modes can switch from "scan to scan" polarity, allowing for as much metabolite detection and identification as possible in a single injection to obtain more proteomic information

Compared with CID, HCD performs cleavage and obtains expected MS/MS spectra, thereby improving identification ability. High spectral quality and wide dynamic range can identify and quantitatively determine proteins that cannot be detected by other instruments. Quantitative/qualitative analysis capabilities enable proteomics discovery experiments to be transformed into highly specific environmental and food analyses for quantitative analysis of target proteins

Due to its specificity surpassing that of triple quadrupole Q-TOF or Q-Trap, this mass spectrometer has become the expected choice data for quantitative/qualitative screening platforms. Its full scan MS/MS capability and HRAM data are stable for each identification, providing stability for quantitative confirmation in clinical research and forensic toxicology.

The combination of SRM and HRAM functions enables quantitative determination of endogenous steroids, prohibited drugs, and analgesics. Application specific software Thermo Scientific for improving flux ™ ExactFinder ™、 LCQuan ™、 Mass Frontier ™、 MetQuest ™、 MetWorks ™、 SIEVE ™ And TraceFinder ™ The software can be used for data analysis.