QuantStudio 3D Digital PCR System

NegotiableUpdate on 09/23

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

Chip based economical digital PCR now allows you to use the new Applied Biosystems ™ QuantStudio ™ 3D digital PCR system eliminates the limitations of real-time fluorescence quantitative PCR

Product Details

Economic digital PCR based on chips

Now, you can use the new Applied Biosystems ™ QuantStudio ™ The 3D digital PCR system eliminates the limitations of real-time fluorescent quantitative PCR. As a simple and economical platform, QuantStudio ® The 3D digital PCR system allows you to obtain accurate and sensitive absolute quantitative data, meeting higher requirements for applications. QuantStudio ® The 3D digital PCR system adopts sealed chip technology, providing a convenient, reliable, and stable digital PCR method. Just need to sample it to complete.

The new generation of PCR technology has arrived

The QuantStudio 3D digital PCR system has the following advantages:

• Simple——Sample loading and running, a chip based turnkey workflow

• Economy——Less than 50% of the competitive platform price

• Absolute quantification——Quantification of concentration as copy number/μ L without the need for a standard curve

Simple workflow, no need for excessive manual operation steps and sample processing.

The QuantStudio 3D digital PCR chip makes the workflow simple and convenient. Unlike other methods that require multiple transfer steps and preliminary sample digestion, the QuantStudio 3D digital PCR chip only requires simple sample addition and encapsulation for PCR analysis. The QuantStudio 3D digital PCR system can simultaneously complete the thermal cycling steps of 24 chips, with a read time of less than 30 seconds for each chip. It uses a simple and simplified workflow to complete digital PCR.

Economic solution enables any laboratory to conduct digital PCR

Due to the fact that the QuantStudio 3D digital PCR system is not based on expensive droplet flow technology, its price is significantly lower than other systems on the market. Even the closest digital PCR competitors require an average of 10 years * of use to save the cost of the QuantStudio 3D digital PCR system. In addition, as over 8 million TaqMen ® We are the main supplier of detection methods and digital PCR, and we can maximize the savings of your experimental needs.

Complete absolute quantification at your fingertips

The absolute quantification of digital PCR is achieved by running thousands of individual reactions in parallel. Applied Biosystems ™ QuantStudio ™ The 3D digital PCR chip is manufactured using a silicon-based substrate and has 20000 uniformly sized nanoscale reaction holes etched on it. Compared with droplet flow based methods, it achieves better sample loading control and less reaction failure probability, ensuring higher accuracy in absolute quantification.

Digital PCR extends the existing TaqMan ® The application scope of detection has achieved higher sensitivity, accuracy, and absolute quantification. This type of application includes rare mutation detection (also known as needle in the haystack detection, such as searching for mutations in cancer samples), quantification and detection of viruses and bacteria (obtaining absolute numbers of pathogens), detection of genetically modified organisms (such as detecting the presence of GMO seeds), optimization of next-generation sequencing workflows (precise quantification of NGS libraries and validation of sequencing results), and reference standard quantification (establishing a reference standard for real-time quantitative experiments).

The number of reactions per run	1 per chip
Module specifications	20000 areas/chip
Color combination (multiple)	2 (Endpoint detection)
runtime	~Read 1 chip in 30 seconds
Programmable volume	Not applicable
Verify volume	14.5 μ L per chip
Automation compatibility	No
Remote Monitoring	No
Onboard memory	Yes,>500 chips
Email notification	No
Assist in meeting the requirements of 21 CFR Part 11	No
sensitivity	As low as 1 copy
dynamic range	5 logs
chemical method	TaqMan and SYBR Green
Scope of application	Rare mutation detection, molecular quantification standards, copy number variations, absolute quantification, pathogen detection, digital PCR
IVD platform	No
Temperature uniformity	Not applicable
excitation light source	OptiFlex system with white LED lights
Filter/Color	3 excitation filters (450-600 nm) and 3 emission filters (500-640 nm)
Thermo Fisher Cloud Services	direct connection
Wi Fi connectivity	yes
size	Width 13.5 cmX Depth 23.25 cmX Height 21 cm